SR. NO TEST TEST COMPONENTS Method Specimen/Transport TAT Clinical Applications
1 Calcoflour Staininig CALCOFLUOR STAININIG Fluorescent microscopy All specimens except Blood 6 hrs Highly sensitive method for detection of Yeast or Fungus by Florescent staining.
2 Chlamydia and N.Gonorrohoeae By Gene expert Chlamydia trachomatis, Neisseria gonorrhoeae RT-PCR by GeneXpert Urine in sterile container, Vaginal swab/ Endocervical swab in transport medium 20-30 ml of Urine or Vaginal or endocervical swab in transport kit 4Hrs Molecular method for detection of N. Gonorrohoeae and C. trachomatis
3 FUNGUS CULTURE Gram stain, AFB stain, KOH, Identification of fungus grown, Colony count Conventional Fungus Culture and Rapid Culture for Yeast. CSF: 2 mL (1 mL min.) in a Red Top (No Additive) tube or sterile screw capped vial. Do not use SST gel barrier tubes. Ship refrigerated. Bone Marrow: 2 mL (0. 5 mL min.) Bone marrow in 1 Green Top (Sodium Heparin) tube. Ship refrigerated. Pus/Body fluids (Pleural/ Pericardial/ Ascitic/ Synovial/ Ocular/ Bronchial washings/BAL). Aspirates /Semen/ Stool: Submit as much as possible (1 mL/1g min.) in sterile screw capped container. Ship refrigerated. Endometrial curettings/Tissue: Submit in sterile normal saline in a sterile screw capped container. Ship refrigerated. Swabs: Submit swabs in 1 mL sterile normal saline in sterile screw capped container. Ship refrigerated. Sputum/Urine: Submit 2 Spot (random) morning samples, 5–10 mL (1 mL min.) sputum/10 mL (5 mL min.) urine in sterile screw capped container. Ship refrigerated. Skin: Submit 2–3 mm scrapings from periphery of skin lesion in sterile container or between 2 sterile glass slides. Wrap slides in black paper. Ship refrigerated. Hair: Submit plucked 10–12 infected, lustreless hair including root in a sterile container. Ship refrigerated. Nail: Submit clippings and powdery material from friable and discolored region of affected nail. Ship refrigerated. 10 Days Detection of fungal infection by culture method.
4 FUNGUS MIC Antifungal susceptibility with MIC value and interpretation of Posaconazole, Amphotericin-B, Fluconazole, Itraconazole, Ketoconazole, Flucytosine, Voriconazole, Caspofungin Broth microdilution Pure growth of Yeast from any sample 72Hrs Susceptibility of Antifungal drugs against any Yeast or Aspergillus spp, by MIC method
5 Gram - Stain GRAM STAIN EXAMINATION : Light Microscopy after gram stain Any specimen except blood/1ml/Sterile container 4Hrs Detection of any microorganism and pus cells by microscopic examination. Gram stain from Vaginal swab for diagnosis of Bacterial vaginosis (Nugent score).
6 IDENTIFICATION BY MALDI TOF (Mass Spectrometry) Identification of organism by MALDI TOF Mass spectrometry/td> Upto 48 hrs of culture growth or any bacteria or yeast. Growth on Blood agar or LJ medium or MGIT in case of NTM or MTB. Hyphal growth on plate or slant. 2Hrs Ultra rapid identification of any Bacteria, Yeast, Hyphal fungus, Mycobacteium spp from growth
7 KOH PREPARATION Light Microscopy Submit Skin, Nail scrapings, Hair ( including root), Cerebrospinal fluid, in a sterile screw capped container OR 2 mL whole blood / bone marrow in 1 Green Top (Sodium Heparin) tube. Ship at 18–22°C. Submit Body fluid, Sputum, Bronchial washings / Tissue in a sterile screw capped container OR Oral/Genital/wound swabs in 0. 5 mL sterile normal saline. Ship refrigerated. 4Hrs Detection of Fungal infection in clinical specimen by Microscopy.
8 PYOGENIC CULTURE AND SENSITIVITY FOR BACTERIA Gram stain, ZN stain, Oragnism isolated, Colony count Aerobic culture and susceptibility repoting of bacteria by Kirby bauer method Any specimen except blood 48-72 hrs Culture of bacteria from specimen along with its identification, colony count and susceptibility by Kirby bauer method.
9 AUTOI (Identification on Vitek Automated identification on Vitek 2 compact system Pure growth of bacteria or yeast on plate or slant Submit pure isolate on appropriate media. Ship refrigerated. DO NOT FREEZE. Specify collection site and provide brief clinical history. 48 Hrs Identification of Bacteria/Yeast by automated system
10 SPECIMEN FOR NEISSERIA GONORRHOEAE BY MICROSCOPY Gram stain, Light microscopy Urethral discharge/ Urine 2 Hrs Detection of N. gonorrheae from urine or urethral discharge by gram staining
11 Chlamydia antigen - Immunochromatographic assay Endocervical swab / Cytology brush 4 Hrs Detection of Chlamydia antigen from Endocervical swab or Cytology brush