| G81 |
AFB Culture (MGIT) |
42 |
0 |
"Liquid rapid culture on MGIT after processing specimen with Modified Petroff's Method of digestion and decontamination. Followed by Differentiation between MTB and NTM/MOTT by TBMPT64Ag Card test." |
Respiratory specimens. Also acceptable: Body fluid, CSF, gastric aspirate, tissue, or urine in sterile leak proof container |
2-10 ml |
2TO8DC |
Identification of NTM and susceptibility testing of all Mycobacteria is performed on positive cultures at an additional charge.s |
| G1933 |
AFB stain for 3 Days |
3 |
|
"Conventional microscopic examination after ZN stain" |
"Sputum or Urine (Early morning specimen) which ever is prescribed for testing. In case of urine, full voided urine is recommended." |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
An AFB Stain alone without culture is appropriate only when following respiratory specimens on previously diagnosed patients. Studies have shown that AFB stain without culture has a poor negative predictive value. For
a higher predictive value, order AFB culture. |
| G1936s |
AFB Stain by IF for 3 Days |
3 |
|
Fluorescence microscopic examination after Auramine O Stain. |
"Sputum or Urine (Early morning specimen) which ever is prescribed for testing. In case of urine, full voided urine is recommended." |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
An AFB Stain alone without culture is appropriate only when following respiratory specimens on previously diagnosed patients. Studies have shown that AFB stain without culture has a poor negative predictive value. For
a higher predictive value, order AFB culture. |
| G1946 |
Anaerobic Culture |
5 |
0 |
"Conventional anaerobic culture and automatic identification on MALDI TOF after growth" |
Aspirate, body fluids, tissues, or material collected from areas without normal anaerobic flora |
|
Ambient temperature |
"Specimen stability at ambient is 48 hour Contact the laboratory prior to collection of the specimen if consultation on collection containers or transport is needed. To perform anaerobic culture from Blood, Bactec Anaerobic
test is suggested." |
| G330 |
Bactec Aerobic Culture |
0 |
72 |
Bactec continuous monitoring and rapid detection system |
"Transfer Blood/Fluid/Pus to a Bactec aerobic resin bottle using aseptic techniques, as defined for blood culture collection. Site of specimen draw is required (e.g. Left Hand, Right Hand, CVP line, Arterial line etc).
Adult minimum: 3 mL blood (aerobic: 8-10 mL recommended)" |
3-10 ml |
Ambient temperature |
Good volume of blood/fluid is crucial for better recovery of organisms. It increases detection chances. Very high volume (Higher than 10 ml) is not recommended as it can further inhibit the growth. |
| G1905 |
Bactec Myco-F Culture |
5 |
0 |
Bactec continuous monitoring and rapid detection system |
"Transfer Blood/Fluid/Pus to a Bactec aerobic resin bottle using aseptic techniques, as defined for blood culture collection. Site of specimen draw is required (e.g. Left Hand, Right Hand, CVP line, Arterial line etc).
Adult minimum: 1 mL blood (aerobic: 1-5 mL recommended)" |
1-5 ml |
Ambient temperature |
Good volume of blood/fluid is crucial for better recovery of organisms. It increases detection chances. Very high volume (Higher than 10 ml) is not recommended as it can further inhibit the growth. |
| G1888 |
Bactec Ped plus culture |
3 |
0 |
Bactec continuous monitoring and rapid detection system |
"Transfer Blood (Pediatric patient) to Bactec Peds Plus resin bottle using aseptic techniques, as defined for blood culture collection. Site of specimen draw is required (e.g. Left Hand, Right Hand, CVP line, Arterial
line etc). Adult minimum: 1 mL blood ( 1-3 mL recommended)" |
1-3 ml |
Ambient temperature |
Good volume of blood/fluid is crucial for better recovery of organisms. It increases detection chances. Very high volume (Higher than 03 ml) is not recommended as it can further inhibit the growth. |
| G2363 |
Biofire Gastrointestinal Panel |
0 |
8 |
Qualitative Polymerase Chain Reaction |
Stool |
1 ml |
Ship refrigerated |
|
| G2316 |
Biofire Meningitis panel |
0 |
8 |
Qualitative Polymerase Chain Reaction |
CSF |
1 ml (Min 0.5 ml) |
2TO8DC |
A negative result does not exclude a diagnosis of meningitis or encephalitis due to infection. |
| G2347 |
Chlamydia and N. Gonorrhoeae detection By GeneXpert CB NAAT |
1 |
0 |
Cartridge based Nucleic Acid Amplification Test |
Vaginal Swab, Rectal Swab, Endocervical swab, Urethral Swab, Genital Swab, Penile Swab or Pharyngeal swab in transport medium or Urine |
Swab Or if urine than 15-20 ml |
2TO8DC |
|
| G2020 |
Clostridium Difficile GDH Ag + Toxin A and B Detection From Stool |
0 |
4 |
Qualitative immunochromatographic assay |
Stool |
Representative portion of feces; 5 mL, Min 1 ml |
Ship refrigerated or frozen |
|
| G2388 |
Colistin MIC by Microbroth dilution |
1 |
0 |
MIC by microbroth dilution |
Pure culture growth on culture plate or slant |
Pure bacterial growth |
Ambient temperature |
As per CLSI guidelines, Polymyxin B antibiotic sensitivity is to be tested and reported only by Microbroth dilution method. |
| G82 |
Fungus Culture |
10 |
0 |
Culture/Identification |
Material or fluid from any body site, except blood |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
"Remarks Additional information required: Specimen source. Notify laboratory if Malassezia furfur is suspected, special media must be used for the cultivation of this yeast" |
| G2000 |
Fungus susceptibility testing by MIC |
3 |
0 |
Broth Microdilution |
Pure growth of yeast on plate or slant |
|
Ambient temperature |
Isolate identification and specimen source required |
| G2084 |
GeneXpert-TB CB-NAAT |
0 |
24 |
Cartridge based Nucleic Acid Amplification Test |
Respiratory specimens. Also acceptable: Body fluid, CSF, gastric aspirate, tissue, or urine in sterile leak proof container |
Minimum 2 ml |
2TO8DC |
"1. Specimen volumes less than indicated may decrease sensitivity of testing., 2. If insufficient volume is submitted, test or tests will be canceled." |
| G2308 |
Identification By Maldi TOF (Mass Spectrometry) |
0 |
2 |
"Mass spectrometry: MALDI (Matrix-Assisted Laser Desorption/Ionization)" |
Pure growth of bacteria or yeast or hyphal fungus on plate or slant |
|
Ambient temperature |
|
| G2011 |
KOH Preparation |
0 |
4 |
Microscopic examination |
"Submit Skin, Nail scrapings, Hair ( including root), Cerebrospinal fluid, in a sterile screw capped container OR 2 mL whole blood / bone marrow in 1 Green Top (Sodium Heparin) tube. Ship at 18–22°C. Submit Body fluid,
Sputum, Bronchial washings / Tissue in a sterile screw capped container OR Oral/Genital/wound swabs in 0. 5 mL sterile normal saline." |
min 0.5 - 1 ml6 |
Ship refrigerated |
Specimen source preferred to be mentioned while sending sample |
| G2422 |
Mycobacterial Identification By Maldi TOF |
2 |
0 |
"Mass spectrometry: MALDI (Matrix-Assisted Laser Desorption/Ionization)" |
Pure growth of Mycobacteria in MGIT tube/LJ slant/Agar plate |
|
Ambient temperature |
|
| G2001 |
NTM / MOTT Drug susceptibility testing by MIC |
10 |
0 |
Microbroth Dilution |
Actively growing isolate in pure culture (MGIT tube/LJ Medium) |
|
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| G2341 |
Pneumoslide IgM Panel (Bacterial + Viral) |
2 |
0 |
Indirect immunofluorescence |
Blood in plain tube (Serum) |
1 ml minimum |
2TO8DC |
|
| G2013 |
Pneumocystic Jirovecii (Carinii) detection |
2 |
0 |
"Giemsa and Methenamine silver stain" |
Respiratory specimen: Bronchial washing, bronchoalveolar lavage (BAL), or induced sputum |
Minimum 2 ml |
2TO8DC |
A negative stain result does not exclude the possibility of infection. False-negative results may occur due to sampling errors or a low number of organisms in the specimen. |
| G2030 |
Primary Stain |
0 |
12 |
"Microscopic examination after various stains like Gram stain, AFB stain, KOH, AFB by IF (Calcofluor white when requested)" |
"Any specimen except blood" |
|
2TO8DC |
|
| G2310 |
Biofire Respiratory Panel |
0 |
8 |
Qualitative Polymerase Chain Reaction |
Nasopharyngeal Swab |
NPS in minimum volume of 1 mL of VTM |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
"Negative results in the setting of a respiratory illness may be due to infection with pathogens that are not detected by this test, or lower respiratory tract infection that may not be detected by an NPS specimen.
Positive results do not rule out coinfection with other organisms." |
| G255 |
Semen Wash By Gradient Method |
0 |
4 |
Semen wash by gradient method |
Semen in sterile container |
|
RT |
"Transport the sample immediately. It should reach laboratory within 30 minutes. Collect the processed sample immediately after report is ready." |
| G2016 |
Neisseria gonorrhoeae detection By Microscopy |
0 |
6 |
Light microscopy |
Urethral discharge, Vaginal or cervical discharge, Urine |
Minimum - 1 ml (In case of discharge) |
Ambient temperature |
|
| G2018 |
Stool For Hanging Drop Preparation |
0 |
4 |
Light Microscopy |
Stool |
Minimum 2 ml |
Ambient temperature |
|
| G2023 |
TB Drug Second Line |
20 |
0 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
Minimum 1 ml |
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2022 |
TB Drug First Line |
20 |
0 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
Minimum 1 ml |
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2002 |
TB Drug Sensitivity (First line and Second line) |
20 |
0 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
Minimum 1 ml |
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2140 |
TBDrug10 |
14 |
14 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
Minimum 1 ml |
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2149 |
TBDrug19 |
14 |
14 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
Minimum 1 ml |
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2134 |
TBDrug4 |
14 |
14 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
|
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2135 |
TBDrug5 |
14 |
14 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
|
2TO8DC |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. |
| G2130 |
Wet Film Examination From Sample |
0 |
4 |
Light Microscopy |
"Body fluid, Urine, CSF" |
Min 1 ml |
RT |
|
| T2598 |
AFB stain |
0 |
4 |
"Conventional microscopic examination after ZN stain" |
All specimens except blood |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
An AFB Stain alone without culture is appropriate only when following respiratory specimens on previously diagnosed patients. Studies have shown that AFB stain without culture has a poor negative predictive value. For
a higher predictive value, order AFB culture. |
| T3894 |
Streptococcus pneumoniae Antigen from CSF |
0 |
4 |
Qualitative Immunochromatography |
CSF in a sterile leak proof container |
Min. 1 ml |
Ship refrigerated or frozen |
Patients who have received the S. pneumoniae vaccines may test positive in the 48 hours following vaccination. Avoid antigen detection testing for at least 7 days after receiving vaccination. |
| T3816 |
1,3-Beta D Glucan Level |
5 |
0 |
Semi-Quantitative Colorimetry (Protease Zymogen-Based Colorimetric Assay) |
Serum - Plain Red or Serum Separator Tube (SST) |
Min 1 ml |
2TO8DC |
|
| T3847 |
Cryptosporidium Antigen From Stool |
0 |
4 |
Rapid Immunochromatographic assay |
Stool |
Minimum 1 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| G328 |
Bactec Anaerobic culture |
5 |
|
Automated blood (BACTEC)culture |
"Transfer Blood/Fluid/Pus to a Bactec anerobic resin bottle using aseptic techniques, as defined for blood culture collection. Site of specimen draw is required (e.g. Left Hand, Right Hand, CVP line, Arterial line etc).
Adult minimum: 3 mL blood (8-10 mL recommended)" |
8-10 ml |
Ambient temperature |
Good volume of blood/fluid is crucial for better recovery of organisms. It increases detection chances. Very high volume (Higher than 10 ml) is not recommended as it can further inhibit the growth. |
| T1119 |
AFB detection by Immunofluorescence |
0 |
6 |
Fluorescence microscopic examination after Auramine O Stain. |
All specimens except blood |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
An AFB Stain alone without culture is appropriate only when following respiratory specimens on previously diagnosed patients. Studies have shown that AFB stain without culture has a poor negative predictive value. For
a higher predictive value, order AFB culture. |
| T718 |
Albert stain |
0 |
3 |
Alberts stain, Light Microscopy |
Throat swab, Respiratory samples |
|
Ambient temperature |
|
| T1383 |
Identification on Vitek |
2 |
0 |
Fluorogenic methodology for organism identification by automated system |
Pure growth of bacteria or yeast on plate or slant |
|
2TO8DC |
Some organisms which are not identified on Vitek, might get identified by MALDI TOF automated system, which is having higher library for identification. |
| T2670 |
Biological indicator |
2 |
0 |
"Indicator growth detection system post incubation" |
Steam or ETO biological indicator |
1 Vial each |
Ambient temperature |
|
| T2403 |
Escherichia coli Antisera O 157 |
|
24 |
Slide agglutination with antisera |
|
|
Ambient temperature |
|
| T705 |
Gram stain |
0 |
6 |
"Light Microscopy after gram stain" |
Any specimen except blood |
Minimum 1 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| T388 |
Indian Ink Preparation |
0 |
3 |
Negative staining |
Cerebrospinal fluid (CSF) or other specimen in sterile container |
0.5 ml Minimum |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| T1380 |
Legionella Pneumophilia Antigen From Urine |
0 |
6 |
Immunochromatographic assay |
Random urine |
1-2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| T1933 |
LPA (Line Probe Assay) MTB-XDR |
3 |
|
The DNA•STRIP technology combines a specific amplification with subsequent reverse hybridization |
Any representative sample (Sputum, pus, fluid or any other clinical sample except blood) |
Minimum 2 ml |
2TO8DC |
"This includes the quinolone-resistance determining region (QRDR) of gyrA (from codon 85 to 96) and of gyrB (from codon 536 to 541) (16) genes for detection of resistance to fluoroquinolones and the rrs (nucleic acid
position 1401, 1402 and 1484) and the eis promoter region (from -37 to -2 nucleotides upstream) for detection of resistance to second line injectable drugs. (Aminoglycosides)." |
| T1936 |
LPA (Line Probe Assay) NTM AS |
3 |
|
The DNA•STRIP technology combines a specific amplification with subsequent reverse hybridization |
Pure growth of NTM in MGIT medium/LJ slant. |
|
2TO8DC |
|
| T3267 |
MIC BY E-TEST |
1 |
|
Epsilometer MIC detection by gradient method |
"Pure growth of Bacteria/Yeast on media plate or slant" |
|
Ambient temperature |
Isolate identification and specimen source required. Individual antibiotics must be specified |
| T684 |
Microbiological surveillance |
2 |
|
"Swab culture and air sample testing by aerobic culture for detection of organism load/type. Air sampling by settle plate technique." |
Maximum 30 swab from different relevant areas with or without maximum 2 air samples |
|
2TO8DC |
It is mandatory to provide list of sites from where swabs or samples are being collected |
| T2590 |
Identification on Vitek (For Pharma) |
3 |
|
Fluorogenic methodology for organism identification in automated system |
Actively growing organism, in pure culture (Slant or plate) |
|
RT |
|
| T2257_1 |
Water Culture for Surveillance |
3 |
|
Water culture testing and reporting by MPN method |
Water in two sterile containers for sufficient volume |
Minimum 60 ml |
2TO8DC |
|
| T2257 |
Food Microbiological Testing |
4 |
|
|
Food in sterile container |
|
2TO8DC |
In case of testing for any specific pathogen, condition or specimen type, it is mandatory to mention same in test request form (TRF). |
| T3893 |
Streptococcus pneumoniae Antigen from Urine |
0 |
6 |
Qualitative Immunochromatography |
Random urine |
Minimum 2 ml |
2TO8DC |
False-positives may occur because of cross-reactivity with other members of S. mitis group. Clinical correlation is recommended.
|
| T3817 |
Rotavirus Antigen From Stool |
0 |
6 |
Immunochromatographic assay |
|
1 ml |
2TO8DC |
|
| T3922 |
Streptococcus A antigen detection from Throat swab |
0 |
6 |
Immunochromatographic assay |
Throat swab in transport medium |
|
Ambient temperature |
|
| T4033 |
Listeria monocytogenes Ag detection form stool |
0 |
6 |
Immunochromatographic assay |
Stool in leak proof container |
Minimum 2 ml |
2TO8DC |
|
| T4035 |
Adenovirus antigen from Respiratory sample |
0 |
6 |
Immunochromatographic assay |
Nasal swab, Nasopharyngeal wash or aspirate. |
|
2TO8DC |
|
| G2448 |
Biofire Pneumonia Plus Panel |
0 |
8 |
Qualitative and Semi Quantitative Polymerase Chain Reaction |
"Bronchoalveolar lavage (BAL)-like specimens • Including BAL and mini-BAL Sputum-like specimens • Including induced and expectorated sputum as well as endotracheal aspirate (ETA) collected according to standard technique" |
1 ml |
2TO8DC |
Negative results in the setting of a respiratory illness may be due to infection with pathogens that are not detected by this test, pathogens below the limit of detection, or in the case of bacterial analytes, present
at levels below the lowest reported 10^4 copies/mL bin. Detection of analytes does not rule out co-infection with other organisms; the agent(s) detected by the FilmArray Pneumonia Panel may not be the definite cause
of disease. |
|
Parasite Identification |
0 |
6 |
|
|
|
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
| G2446 |
Genex Carba R Xpert Assay |
0 |
6 |
Qualitative PCR |
"1. Pure culture of carbapenem-non-susceptible organism or 2. Perirectal or Rectal Swab" |
|
RT |
False-negative results may occur due to inhibition of polymerase chain reaction, sequence variability underlying primers and probes, or the presence of the genes in quantities lower than the limit of detection of the
assay.
|
|
COVID NAAT |
0 |
6 |
Cartridge based Nucleic Acid Amplification Test |
"Nasopharyngeal swab in Viral transport medium Nasopharyngeal aspirate or nasal washings in sterile container" |
Min 2 mL of VTM and If Aspirate - 1.5 ml |
2TO8DC |
Detected results are indicative of the presence of SARS-CoV-2 RNA. Clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. |
|
TBPZA (Pyrazinamide susceptibility for MTB) |
20 |
0 |
Indirect method of Antitubercular testing by Bactec MGIT 960 system |
Pure growth of Mycobacterium tuberculosis complex in MGIT tube |
|
2TO8DC |
"Pure growth of Mycobacterium tuberculosis complex in MGIT tube is preferable sample. In case of growth on any other medium or old growth, re-culturing is required which may take additional charges. When this test is
ordered, the additional test will always be performed and charged separately." |
|
Giardia lamblia Antigen From Stool |
0 |
4 |
Immunochroatographic assay |
Stool in leak proof container |
Minimum 2 ml |
2TO8DC |
|
|
SPECIAL CULTURE |
5 |
0 |
"Selective/ Enriched culture of required organism" |
Any specimen suitable for detection of ordered organism detection |
Minimum 2 ml |
Refrigeration recommended. Ambient temperature if sample reaches the lab within 72 hrs from the time of complete collection. |
|
|
XPERT HCV (HEPATITIS C VIRUS) VIRAL LOAD |
0 |
24 |
Real time Reverse transcriptase PCR |
"Whole blood should be collected in K2-EDTA tubes, EDTA-PPT or serum collection tubes. It is mandatory to Collect 2 tubes to maintain sufficiency of the sample." |
1.2 mL plasma or serum |
2TO8DC |
|
| 2033 |
Biofire Blood Culture ID Panel |
0 |
8 |
Qualitative Polymerase Chain Reaction |
Blood culture |
1 ml |
RT |
"Positive results do not rule out co-infection with organisms not included in the BioFire BCID2 Panel. " |
|
Polymyxin-B MIC by Microbroth dilution |
1 |
0 |
MIC by microbroth dilution |
Pure culture growth |
|
Ambient temperature |
As per CLSI guidelines, Polymyxin B antibiotic sensitivity is to be tested and reported only by Microbroth dilution method. |
| G2032 |
GeneXpert-MTB/XDR |
0 |
24 |
Cartridge based Nucleic Acid Amplification Test |
"Respiratory specimens. Also acceptable: Body fluid, CSF, gastric aspirate, tissue, or urine in sterile leak proof container Pure MTB isolate in a proper screw cap, sterile tube with proper packaging." |
Minimum 2 ml |
2TO8DC |
"Specimen volumes less than indicated may decrease sensitivity of testing." |
