Histopathology
| SR NO | TEST | TEST COMPONENTS | METHOD | SPECIMEN/TRANSPORT | TAT | CLINICAL APPLICATIONS |
|---|---|---|---|---|---|---|
| 1 | Special stains | PAS stain PASM stain MT stain Congo-red stain |
Manual Sections cut on microtome at various thickness as required for special stain |
Formalin fixed tissue/paraffin blocks of tissue | 24 hrs | For definite diagnosis of clinically suspected on routine H &E stain. |
| 2 | Immunofluorescence study | IgG, IgA, IgM, C3, C1q, Kappa, Lambda, Fibrinogen Albumin |
Sections cut on cryostate | Phosphate buffer saline or Michell media Trnasport with coolent |
24 hrs | For further typing of glomerular disease diagnosed on routine H &E stain |
| 3 | Immunohistochemistry study. | IgA, SAA IgG4 Kappa Lambda C4d |
Sections cut on microtome at various thickness | Formalin fixed tissue/paraffin blocks of tissue | 24 to 48 hrs | For definitive diagnosis of IgA nephropathy if IF can not be done with IgA, for IgG4 disease and for differentiating porimary from secondary amyloidosis. |
| 4 | Electron Microscopy | Outsourced | Ousourced to Jaslok hospital | Gluteraldehyde with coolent | 10-12 days | For detection of diseases liked thin basement membrane disease, Fibrillary disease, DDD etc |
| SR NO | TEST | TEST COMPONENTS | METHOD | SPECIMEN/TRANSPORT | TAT | CLINICAL APPLICATIONS |